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Table 2 Western blot analysis of protamines in mice with NPYq deficiencies

From: Deficiency in mouse Y chromosome long arm gene complement is associated with sperm DNA damage

  Mean band intensityb (±SEM) representing mature protamine forms   
Mice examineda Coomassie Hub2B (protamine 2) Mice examineda Mean band intensityb (±SEM) representing immature protamine 2 (PreP2)
2/3NPYq- (n = 7) 141.9 ± 4.6 125.3 ± 4.6 - -
XYRIII (n = 6) 138.2 ± 5.0 124.9 ± 5.0   
9/10NPYq- (n = 7) 82.5 ± 4.8* 43.5 ± 0.3*** 9/10NPYq- (n = 5) 89.2 ± 4.5**
XYTdym1Sry (n = 6) 131.0 ± 5.2 49.3 ± 0.3 XYTdym1Sry (n = 4) 53.8 ± 5.0
NPYq-2 (n = 3) 22.1 ± 16.2 20.8 ± 6.6   
XYRIII (n = 3) 76.0 ± 16.2 36.9 ± 6.6 - -
  1. aAt least two separate gels were run for each NPYq deficient genotype (2/3NPY-, two gels; 9/10NPYq-, four gels; NPYq-2, two gels). For PreP2, data were obtained from only two gels. bThe data were analyzed by two-way ANOVA with gel and genotype as factors, from which the genotype means and errors are derived. Statistical significance: *P < 0.005; **P < 0.0005; ***P < 0.00005. The lack of statistical significance for the reduction observed in NPYq-2 was almost certainly due to the limited number of samples. SEM, standard error of the mean.