Table 2 Correlation between differential protein expression by 2DGE (protein ratio) and differential gene expression by microarray (mRNA ratio)
From: A mouse embryonic stem cell bank for inducible overexpression of human chromosome 21 genes
Spot ID* | Gene | Protein name | Protein ratio 48 h/0 h* | mRNA ratio 24 h/0 h |
|---|---|---|---|---|
3214 | Uchl1 | Ubiquitin carboxy-terminal hydrolase L1 | 0.58 | 0.63 |
662 | Dppa4 | Developmental pluripotency associated 4 isoform 1 | 0.65 | 0.75 |
3549 | Igf2bp2 | Insulin-like growth factor 2 mRNA-binding protein 2 (IGF-II mRNA-binding protein 2) (IMP-2) | 0.65 | 1.39 |
2836 | Sipa1l1 | Signal-induced proliferation-associated 1-like protein 1 | 0.74 | 1.54 |
425 | Lap3 | Cytosol aminopeptidase (Leucine aminopeptidase) (LAP) (Leucyl aminopeptidase) (Leucine aminopeptidase 3) (Proline aminopeptidase)(Prolyl aminopeptidase) | 0.76 | 0.72 |
2512 | Hspd1 | 60 kDa heat shock protein, mitochondrial precursor (Hsp60) (60 kDa chaperonin) (CPN60) (Heat shock protein 60) (HSP-60) (Mitochondrial matrix protein P1) (HSP-65) | 0.78 | 0.75 |
3410 | Eif1a | Eukaryotic translation initiation factor 1A, Y-linked | 0.8 | 0.76 |
403 | Pkm2 | Pyruvate kinase isozyme M2 | 1.26 | 3.05 |
935 | Bdh1 | D-beta-hydroxybutyrate dehydrogenase, mitochondrial precursor | 1.31 | 1.53 |
645 | Serpinh1 | Serine (or cysteine) proteinase inhibitor, clade H, member 1 | 1.44 | 1.36 |
2920 | Ldhb | Lactate dehydrogenase 2, B chain | 1.46 | 0.64 |
3562 | Cotl1 | Coactosin-like 1 | 1.62 | 1.39 |
3653 | S100a11 | S100 calcium binding protein A11 (calizzarin) | 1.89 | 1.18 |
3144 | Sept1 | Septin 1 | 2.28 | 0.71 |
1134 | Gsto1 | Glutathione S-transferase omega 1 | 2.77 | 3.73 |
3588 | Fabp3 | Fatty acid binding protein 3, muscle and heart | 3.05 | 2.2 |
3078 | Apoe | Apolipoprotein E | 3.28 | 0.8 |