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Table 2 Correlation between differential protein expression by 2DGE (protein ratio) and differential gene expression by microarray (mRNA ratio)

From: A mouse embryonic stem cell bank for inducible overexpression of human chromosome 21 genes

Spot ID* Gene Protein name Protein ratio 48 h/0 h* mRNA ratio 24 h/0 h
3214 Uchl1 Ubiquitin carboxy-terminal hydrolase L1 0.58 0.63
662 Dppa4 Developmental pluripotency associated 4 isoform 1 0.65 0.75
3549 Igf2bp2 Insulin-like growth factor 2 mRNA-binding protein 2 (IGF-II mRNA-binding protein 2) (IMP-2) 0.65 1.39
2836 Sipa1l1 Signal-induced proliferation-associated 1-like protein 1 0.74 1.54
425 Lap3 Cytosol aminopeptidase (Leucine aminopeptidase) (LAP) (Leucyl aminopeptidase) (Leucine aminopeptidase 3) (Proline aminopeptidase)(Prolyl aminopeptidase) 0.76 0.72
2512 Hspd1 60 kDa heat shock protein, mitochondrial precursor (Hsp60) (60 kDa chaperonin) (CPN60) (Heat shock protein 60) (HSP-60) (Mitochondrial matrix protein P1) (HSP-65) 0.78 0.75
3410 Eif1a Eukaryotic translation initiation factor 1A, Y-linked 0.8 0.76
403 Pkm2 Pyruvate kinase isozyme M2 1.26 3.05
935 Bdh1 D-beta-hydroxybutyrate dehydrogenase, mitochondrial precursor 1.31 1.53
645 Serpinh1 Serine (or cysteine) proteinase inhibitor, clade H, member 1 1.44 1.36
2920 Ldhb Lactate dehydrogenase 2, B chain 1.46 0.64
3562 Cotl1 Coactosin-like 1 1.62 1.39
3653 S100a11 S100 calcium binding protein A11 (calizzarin) 1.89 1.18
3144 Sept1 Septin 1 2.28 0.71
1134 Gsto1 Glutathione S-transferase omega 1 2.77 3.73
3588 Fabp3 Fatty acid binding protein 3, muscle and heart 3.05 2.2
3078 Apoe Apolipoprotein E 3.28 0.8
  1. Only two gene/protein pairs, apoE and Sept1, showed bifurcated regulation in protein and microarray assays.