Figure 2
From: Characterizing regulatory path motifs in integrated networks using perturbational data
Randomization procedure. (a), (b) shows a hypothetical example of an integrated network of transcriptional links (red), with nodes 1, 4, 10, 12 and 20 being transcription factors, and protein-protein interactions (blue) with one hub protein (node 11). Observed perturbed genes (magenta) when a transcription factor is deleted or overexpressed (node 1, red) is shown on the left (a) and a randomized perturbed data set with the same integrated network is shown on the right (b). With respect to the background distribution of 10,000 such random samples from real data, the TRI-TRI regulatory path (c) is overrepresented as the observed value (red dot) lies at far right tail of the distribution (green curve), while the PPI-PPI-TRI regulatory path (d) is not overrepresented as the observed value lies well within the random distribution.