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Table 2 Quantitative analysis of female fibroblast RNA-FISH data

From: Activity map of the tammar X chromosome shows that marsupial X inactivation is incomplete and escape is stochastic

  Percent female nuclei with
Genes on BACs or fosmids 2 signals 1 signal 0 signals
G6PD , IKBKG 17.0 82.0 1.0
TMLHE 29.0 68.0 3.0
RPL10X 27.0 71.0 2.0
MECP2X , IRAK1 , TMEM187 41.0 54.0 5.0
HCFC1X 7.0 91.0 2.0
AR 9.0 87.0 4.0
PSMD10 6.7 93.3 0.0
STAG2 6.7 92.0 1.3
HPRT, PHF6X 23.5 73.5 3.1
UPF3B 11.7 86.7 1.7
PGK1 18.5 80.4 1.1
ATRX 59.8 39.2 1.0
RBMX 11.0 87.0 2.0
UBA1, RBM10 67.8 32.2 0.0
TBC1D25, GATA1 14.3 84.7 1.0
GATA1, WDR13 14.9 81.3 3.7
GLA, GLRA4 7.5 89.2 3.2
KDM5C 35.0 65.0 0.0
HUWE1X 15.0 85.0 0.0
AKAP4 44.1 51.0 4.9
AMOT 23.0 71.7 5.3
LRCH2 5.2 92.8 2.1
WDR44 14.3 81.6 4.1
  1. Frequency of nuclei with 2, 1 or 0 signals for each X-borne locus. At least 100 nuclei were scored. Only nuclei with two signals for the autosomal control gene, and therefore diploid, were scored. Male efficiencies were used to calculate the expected frequency of nuclei with two signals, one signal and no signal for each test gene. Expected frequencies of cells with two signals were in excess of 90% for all loci, and observed frequencies were significantly different from these expected frequencies in every case (Chi-square test with 2 degrees of freedom). Genes highlighted in bold were used in sequential RNA-DNA FISH experiments.