Figure 5

Genes at the nuclear membrane are inactive. (a) Expression level of genes within subdomains or gaps in mixed-stage embryos. Genes were categorized based on the size of gaps where they reside: extra large gap (XL), >1 Mb; large gap (L), 100 kb to 1 Mb; medium gap (M), 10 to 100 kb; and small gap (S), <10 kb. Box plot representation and the statistical analysis are according to Figure 3a. (b) Expression status during development for transcripts undetectable in early embryos. Transcripts were categorized in LEM-2 subdomains (top), large/medium/small gaps (middle) or extra large gaps (bottom) based on their start coordinates. We defined transcripts that were undetectable in early embryos as those with RNA-seq dcpm (depth of coverage per base per million reads) equals 0 in early embryos. E Emb, early embryo; L Emb, late embryo; L, larva stage; Adult, young adult.