Figure 6From: Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transpositionLibrary complexity. Library complexity for each library shown by incremental, random sampling of 50,000 reads, without replacement, and plotting (on log-log scale) the number of uniquely occurring read-pairs with respect to total number of sampled read-pairs. Species: DM, Drosophila; EC, E. coli; NA18507 and YH1, human. Methods for fragmentation: End., endonuclease; Son., sonication; Tr., transposase. Size selection ranges are given for the YH1 libraries (all these were generated using transposase. Libararies ending in "2" are replicate libraries. 100% uniqueis in gray, i.e. the distribution if there were no duplicates of any sort.Back to article page