Filtration cascade module. (a) The average percentage of candidates identified by the fusion detection module that are removed by each filter is reported. The labels also depict the order the filters have been applied in this case (counter-clockwise starting from the RepeatMasker filter), but it is worth noting that the order of the application of the filters does not affect the final list of candidates. (b) RESPER (ratio of empirically computed SPERs) versus depth of sequencing. The plot shows the RESPER values for SLC45A3-ERG, a real fusion transcript, and P4HB-KLK3, an artifact likely created by the random pairing due to the high expression of KLK3 at different sequencing depths.