EAR amplification protocol in RNA from total blood. (a) BLAST analysis for Alu repeat distribution in the human genome. Alu repeat sequences are present in the UTRs of many genes. The small blue points indicate the homologous sequences at the Alu human consensus repeat. (b) Alu human interspersed repetitive sequence and primers used in this study for EAR amplification. (c) Cq of EAR mRNA plotted against the log of the relative initial amount of the pooled cDNA. (d) Melt-curve diagram generated from the amplification as described in (c).