Figure 5

FISH analysis of the most frequent N. furzeri GRZ tandem repeats. (a) The most abundant, G+C-rich minisatellite, which is comprised of 77-nucleotide monomers, is found in centromeric regions of most chromosomes. (b) The second most abundant G+C-rich minisatellite, which is comprised of 49-nucleotide monomers, also forms centromeric regions of many chromosomes. (c) A G+C-rich, 24-nucleotide minisatellite specifically stains centromeric regions of two chromosome pairs. (d) The most frequent G+C poor satellite, which is comprised of 348-nucleotide monomers, maps to centromeric regions of many chromosomes. Panels on the right side show corresponding DAPI images to better illustrate the staining of centromeric regions. Arrows highlight selected distinct DAPI dull centromeric regions.