ChIP-qPCR analysis of H3K9ac and H3K9m3 association with the tiled regions of indicated genes. Experiments are based on the same cell batches as those examined by microarray. Data are expressed as log2 of fold-enrichment relative to input (mean ± standard deviation) in three independent experiments. Promoter regions covered by amplicons are shown in Figure 3b, bottom panels. The expression pattern of each gene was determined in duplicate transcriptome analysis of NCCIT cells using Affymetrix U133A GeneChips (not shown).