Plant polycistronic precursors containing non-homologous microRNAs target transcripts encoding functionally related proteins

Table 2 Summary of targets predicted for non-homologous putative polycistronic clustered miRNAs in Arabidopsis, rice and poplar

		Targets
Non-homologous *MIRNA*clusters	ESTs overlapping the cluster	Predicted	Cleavage validated for at least one target [reference]*
Arabidopsis
Ath-MIR397b-857	No	Laccases (3/1)	Yes [15, 49, 50]
Ath-MIR859-774	No	F-box proteins (35/5)	Yes [15, 48]
Ath-MIR842-846	No	JR/MBP (1/10)	Yes [15]
Ath-MIR851-771	No	EIF2 for miR771 (1) no target for miR851	No [15]
Ath-MIR850-863	EG495879 EG495880 EG514601 DR380439, AV523115^†	No target	-
Poplar
Ptc-MIR482-1448	DB893204 DB893031 BP928209 CX175581	Disease resistance proteins (12/2)	Yes [44]
Ptc-MIR1446b-477a	No	Gibberellin response modulator-like protein (DELLA) (1)	Yes [43, 44]
Ptc-MIR1446a-477b	No	Gibberellin response modulator-like protein (DELLA) (1)	Yes [43, 44]
Rice
Osa-MIR1876-1862d-1884b	Os10g0170200^‡	No target	-
Osa-MIR1423-1868	Os04g32710^‡	No target	-

For predicted targets, the number of targets found for each miRNA of the non-homologous cluster is indicated in parentheses. *Validated using 5' RACE (rapid amplification of cDNA ends) PCR. ^†ESTs cover the region including only miR863. ^‡The consecutive miRNAs are predicted in the intron of a protein coding gene (putative ribosomal protein). Ath, A. thaliana; Osa, O. sativa; Ptc, P. trichocarpa.

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