Figure 8

Candidate blood transcriptional markers retain their discriminatory power in an additional secondary validation set. (a) Patients with sepsis clustered in region R5 of the training set (comprising eight patients with melioidosis (pink rectangles) and six patients with sepsis caused by other organisms (green rectangles) were hybridized to Illumina Human HT-12 V3 BeadChips and used for microarray analysis as before. The 37 blood transcriptional markers identified from the same samples using Illumina Human V2 BeadChips were used for class prediction analysis of the new dataset in a leave-one-out cross-validation scheme as before. The 37 classifiers discriminated training set samples analyzed using the novel data with 100% accuracy as before, despite the change of microarray platform. (b) The performance of the 37 predictor genes was then further evaluated in a secondary independent test set also analyzed using Illumina Human HT-12 V3 BeadChips. This second independent test set (n = 15) comprised eight patients with melioidosis (pink rectangles) and seven patients with sepsis caused by other organisms (green rectangles). The predictors correctly classified 12 of the 15 samples (80% accuracy).