Table 1 Primer and probe sequences
Target gene (application) | Primers | Probes |
|---|---|---|
seryl-tRNA synthesase (real-time PCR) | F: TATCATCTCAACAGGTATCTACATCTCCTA | NA |
| Â | R: TTTGAGAGTTACATGTGGTATCATCTTTT | Â |
β-tubulin (real-time PCR) | F: CGTGCTGGCCCCTTTG | NA |
| Â | R: TCCTGCACCTGTTTGACCAA | Â |
var2csa DBL2x (real-time PCR) | F: AATCATGGTGGAACACGAACAA | 6-FAM-ATATTTGGTTAGCAATGAA-MGB (allele 1) |
| Â | R: AACTACTACCACTACCAGTGACACTACCAT | 6-VIC-ATATATTTGGACAGCAATGA-MGB (allele 2) |
var2csa DBL4ε (real-time PCR) | F: TATACCGTAAAAGTAACAAAGAATCGGAA | 6-FAM-ATGATTATGGAACCTACAGTC-MGB (allele 1) |
| Â | R: ACAACTACAACAAATGTAGTTCCCATTAATT | 6-VIC-ATGATTATGGCACCTACAGT-MGB (allele 2) |
Pf332 S326P (real-time PCR) | F: AGTGGTTCCCAAGAAGAAGAACAA | 6-FAM-ATGATGTGTCACATAGGA-MGB (wild type) |
| Â | R: ACATTCTGTCATCTAATTTATCCAGTAGAGAT | 6-VIC-ATGTGCCACATAGGA-MGB (Mutant) |
var2csa nest 1 (single cell PCR) | F: ACTTGAAAATGTGTGCAAAGGAGTA | NA |
| Â | R: TTACCCAGTGGAGACGGAACAT | Â |
var2csa lr1 (DNA-FISH/Southern blot) | F: AAAATTACTGTGAATCATTCAGAT | NA |
| Â | R: AAGGTGTTTCAGACGAAGTATTAGCAT | Â |
var2csa lr2 (DNA-FISH) | F: ACTGTGAATGTTACGAATTGTGGATAA | NA |
| Â | R: ACATTTCCCCCTCAATTCCTTT | Â |
var2csa PFHG_05046.1_Sp6_a (RNA-FISH, detecting mRNA) | F: TCATTTAGGTGACACTATAGAAGTGTATATTTT GGGTCATCATTTCGATATTT | NA |
| Â | R: GAGATGAAAACAAGTGTGAAACAAAATC | Â |
var2csa PFHG_05046.1_Sp6_s (RNA-FISH, detecting antisense) | F: TCATTTAGGTGACACTATAGAAGAGATGAAAA CAAGTGTGAAACAAAATCC | NA |
| Â | R: TGTATATTTTGGGTCATCATTTCGAT | Â |
var2csa PFHG_05155.1_Sp6_a (RNA-FISH, detecting mRNA) | F: TCATTTAGGTGACACTATAGAAGTGCTTATAA TTTTCATCATCATTTGGATATTTATC | NA |
| Â | R: CAGATCAATTGAAAGGTTCTAACATTTT | Â |
var2csa PFHG_05155.1_Sp6_s (RNA-FISH, detecting antisense) | F: TCATTTAGGTGACACTATAGAAGAGATCAATT GAAAGGTTCTAACATTTTAGAAC | NA |
| Â | R: CTTATAATTTTCATCATCATTTGGATATTTATC | Â |
kahrp_Sp6_a (RNA-FISH, detecting mRNA) | F: TCATTTAGGTGACACTATAGAAGAGGTTGGTG AACCTGTGGTGCTT | NA |
| Â | R: AACTTTAGCACAAAAGCAACATGAA | Â |
kahrp_Sp6_s (RNA-FISH, detecting antisense) | F: TCATTTAGGTGACACTATAGAAGAGAACTTTA GCACAAAAGCAACATGAA | NA |
| Â | R: GTTGGTGAACCTGTGGTGCTT | Â |