Figure 1

var2csa and Pf332 alleles in P. falciparum genomes and discriminative assay design. Alignments of var2csa and Pf332 gene sequences gathered from the fully assembled 3D7 genome and the partially assembled FCR3, Dd2 and HB3 genomes used for allelic discriminative assays. Accession numbers or genome sequence contigs with strain names within parentheses are presented as a means of identification for all alleles. Assays were designed towards two different parts of var2csa, (a) DBL2x and (b) DBL4ε, and (c) to exon 1 of Pf332. Designed real-time PCR primers are indicated in light blue and probes for allele 1/wild type in green and allele 2/mutant in red. Discriminative SNPs are marked with asterisks.