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Table 7 KOG-assigned EST clusters predicted to be highly 2N-specific based on statistical comparison of libraries

From: Transcriptome analysis of functional differentiation between haploid and diploid cells of Emiliania huxleyi, a globally significant photosynthetic calcifying cell

Cluster ID Number of 2N ESTs P-value Homolog ID Homolog description BLAST
Carbohydrate transport and metabolism      
   GS00451 7 3.9 × 10-3 PIP25_ARATH Probable aquaporin PIP2-5 1 × 10-34
   GS00433 8 1.9 × 10-3 F26_RANCA 6PF-2-K/Fru-2,6-P2ASE liver/muscle isozymes 3 × 10-40
Cell wall/membrane/envelope biogenesis      
   GS01290 8 1.9 × 10-3 ASB3_BOVIN Ankyrin repeat and SOCS box protein 3 (ASB-3) 9 × 10-06
Chromatin structure and dynamics      
   GS02435 6 7.8 × 10-3 H4_OLILU Histone H4 8 × 10-33
Cytoskeleton      
   GS00171 6 7.8 × 10-3 EXS_ARATH Leucine-rich repeat receptor protein kinase EXS precursor 1 × 10-08
Energy production and conversion      
   GS00763 6 7.8 × 10-3 QORH_ARATH Putative chloroplastic quinone-oxidoreductase homolog 6 × 10-25
   GS01632 7 3.9 × 10-3 CYPD_BACSU Probable bifunctional P-450/NADPH-P450 reductase 1 2 × 10-43
General function prediction only      
   GS00580 9 9.7 × 10-4 YMO3_ERWST Uncharacterized protein in mobD 3' region 6 × 10-07
   GS02524 7 3.9 × 10-3 RKIP Raf kinase inhibitor protein (RKIP), Phosphatidylethanolamine-binding protein (PEBP) 1 × 10-06
Inorganic ion transport and metabolism      
   GS00463 8 1.9 × 10-3 NCKXH_DROME Probable Na+/K+/Ca2+ exchanger CG1090 1 × 10-22
   GS05051 7 3.9 × 10-3 B3A2_RAT Anion exchange protein 2 (AE2 anion exchanger) 8 × 10-14
Intracellular trafficking, secretion, and vesicular transport      
   GS02941 9 9.7 × 10-4 STX1A_CAEEL Syntaxin-1A homolog 2 × 10-19
Lipid transport and metabolism      
   GS00955 7 3.9 × 10-3 S5A1_MACFA 3-oxo-5-alpha-steroid 4-dehydrogenase 1 3 × 10-54
Posttranslational modification, protein turnover, chaperones      
   GS06447 6 7.8 × 10-3 CLPP3_ANASP Probable ATP-dependent Clp protease proteolytic subunit 3 2 × 10-31
   GS02029 8 1.9 × 10-3 UBCY_ARATH Ubiquitin-conjugating enzyme E2-18 kDa 4 × 10-20
   GS03925 8 1.9 × 10-3 FKBP4_DICDI FK506-binding protein 4 (peptidyl-prolyl cis-trans isomerase) 1 × 10-07
Replication, recombination and repair      
   GS00109 8 1.9 × 10-3 MCM2_XENTR DNA replication licensing factor mcm2 1 × 10-109
Secondary metabolites biosynthesis, transport and catabolism      
   GS00417 6 7.8 × 10-3 WBC11_ARATH White-brown complex homolog protein 11 9 × 10-28
Signal transduction mechanisms      
   GS00826 6 7.8 × 10-3 STK4_BOVIN Serine/threonine-protein kinase 4 2 × 10-47
   GS00712 7 3.9 × 10-3 PI4K_DICDI Phosphatidylinositol 4-kinase 3 × 10-43
   GS00083 7 3.9 × 10-3 SHKE_DICDI Dual specificity protein kinase shkE 9 × 10-22
   GS01230 7 3.9 × 10-3 PP2Cc Serine/threonine phosphatases, family 2C, catalytic domain 2 × 10-08
  1. Only clusters with zero ESTs originating from the 1N library are shown. The number of 2N EST reads in each cluster and the P-value for significance of the difference between libraries are shown. Homolog IDs are marked as in Table 5. Clusters are arranged by KOG class. Clusters in bold were chosen for RT-PCR validation. Additional data file 5 gives a complete list of all clusters predicted to be 2N-specific by statistical comparison of libraries.