Figure 6

Changes in H3K9ac, Pol II and H3K36me3 upon stimulation of EL-4 T cells. (a) ChIP assays were performed with antibodies against H3K9ac using unstimulated EL-4 T cells (grey bars), and cells that were stimulated with P/I for 0.5 h (hatched bars), 1 h (white bars), 2 h (dotted bars) and 4 h (black bars). The data for the promoter region of each gene are presented as a ratio of H3K9ac/H3 levels. The mean and standard error of at least three independent experiments are shown. (b, c) ChIP was performed with antibodies against the CTD repeat of Pol II using unstimulated EL-4 T cells (grey bars), and cells that were stimulated with P/I for 0.5 h (lined bars), 1 h (white bars), 2 h (dotted bars) and 4 h (black bars). The data are presented as a ratio of immunoprecipitated DNA to the total input DNA and show Pol II occupancy for the promoter region (primer set D) and 2 kb downstream of the TSS (primer set E). The mean and standard error of three independent experiments are shown for each primer set. (d) A ChIP assay was performed with antibodies against H3K36me3 using unstimulated EL-4 T cells (grey bars), and cells that were stimulated with P/I for 2 h (lined bars) and 4 h (black bars). The data are presented as ratios of immunoprecipitated DNA to the total input DNA and shows H3K36me3 occupancy at the promoter region (primer set D) and 2 kb downstream (primer set E). The mean and standard error of four independent experiments are shown. PMA, phorbol myristate acetate.