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Table 4 RT-qPCR validation of SAGE profile for P1.5 versus adult stages

From: Molecular networks involved in mouse cerebral corticogenesis and spatio-temporal regulation of Sox4 and Sox11 novel antisense transcripts revealed by transcriptome profiling

    Fold change
SAGE tag RefSeq accession Gene ID Adult/P1.5 (SAGE) Adult/P1.5 (RT-qPCR)
aaattattgggaaatcc NM_011123 proteolipid protein (myelin) 1, Plp1 38.9 43.33
tttcagcagtgttggct NM_013556 hypoxanthine guanine phosphoribosyl transferase 1, Hprt1 6.99 2.26
actcggagccaccagac NM_009790 calmodulin 1, Calm1 4.57 1.83
gcttcgtccacacagcg NM_010777 myelin basic protein, Mbp As 1.79
tccccgtcat NM_026106 down-regulator of transcription 1, Dr1 Ps -1.46
gggaaactaagggagag NM_172503 zinc finger, SWIM domain containing 4, Zswim4 -42.42 -1.96*
gaacgcaagttcagccc NM_031404 actin-like 6B, Actl6b -5.51 -2.26
gtgaaactaaaaaaaaa NM_009094 ribosomal protein S4, X-linked, Rps4x -4.72 -2.27
Nil NM_009726 ATPase, Cu++ transporting, alpha polypeptide, Atp7a Nil -2.91
agaagtgtttggagttt NM_008253 high mobility group box 3, Hmgb3 -20.99 -3.53
cctttgtgacagtggcc NM_025635 ZW10 interactor, Zwint 4.65 -4.04
acagtctatgttggagg BQ177886/NM_010487 C57BL/6 whole brain E15.5 (or known as embryonic lethal, abnormal vision, Drosophila-like 3 (Hu antigen C), Elavl3) -41.99 -4.06
gatacttggaatgacta NM_007393 actin, beta, cytoplasmic, Actb -18.49 -4.35
ctggcttctt NM_008538 myristoylated alanine rich protein kinase C substrate, Marcks Ps -4.76
Nil NM_025958 cullin-associated and neddylation-dissociated 2 (putative), Cand2 Nil -5.62
gctttgactgttctctt NM_009870 cyclin-dependent kinase 4, Cdk4 -56.55 -5.77
ctcagtaatg AA122503 EST from M2 cells of skin melanoma -12.28 -11.12
tggagcgttggctgtat NM_009238 SRY-box containing gene 4, Sox4 -31.51 -11.74
cacgacaccccccaccc NM_007792 cysteine and glycine-rich protein 2, Csrp2 Ps -26.10
tgtgtgaggtgttgtga NM_009559 zinc finger protein 57, Zfp57 -16.33 -32.74
gggacctcgtggaagcc NM_010025 doublecortin, Dcx -24.56 -82.11
  1. All RT-qPCR data are statistically significant at P < 0.001 unless specified: * P < 0.01. A disagreement between RT-qPCR and SAGE data. As: adult-specific expression; Nil: SAGE data not available; Ps: P1.5-specific expression. Fold change values of <1.0 are presented in a negative fold change format.