Table 4 RT-qPCR validation of SAGE profile for P1.5 versus adult stages
| Â | Â | Â | Fold change | |
|---|---|---|---|---|
SAGE tag | RefSeq accession | Gene ID | Adult/P1.5 (SAGE) | Adult/P1.5 (RT-qPCR) |
aaattattgggaaatcc | NM_011123 | proteolipid protein (myelin) 1, Plp1 | 38.9 | 43.33 |
tttcagcagtgttggct | NM_013556 | hypoxanthine guanine phosphoribosyl transferase 1, Hprt1 | 6.99 | 2.26 |
actcggagccaccagac | NM_009790 | calmodulin 1, Calm1 | 4.57 | 1.83 |
gcttcgtccacacagcg | NM_010777 | myelin basic protein, Mbp | As | 1.79 |
tccccgtcat | NM_026106 | down-regulator of transcription 1, Dr1 | Ps | -1.46 |
gggaaactaagggagag | NM_172503 | zinc finger, SWIM domain containing 4, Zswim4 | -42.42 | -1.96* |
gaacgcaagttcagccc | NM_031404 | actin-like 6B, Actl6b | -5.51 | -2.26 |
gtgaaactaaaaaaaaa | NM_009094 | ribosomal protein S4, X-linked, Rps4x | -4.72 | -2.27 |
Nil | NM_009726 | ATPase, Cu++ transporting, alpha polypeptide, Atp7a | Nil | -2.91 |
agaagtgtttggagttt | NM_008253 | high mobility group box 3, Hmgb3 | -20.99 | -3.53 |
cctttgtgacagtggcc | NM_025635 | ZW10 interactor, Zwint | 4.65 | -4.04†|
acagtctatgttggagg | BQ177886/NM_010487 | C57BL/6 whole brain E15.5 (or known as embryonic lethal, abnormal vision, Drosophila-like 3 (Hu antigen C), Elavl3) | -41.99 | -4.06 |
gatacttggaatgacta | NM_007393 | actin, beta, cytoplasmic, Actb | -18.49 | -4.35 |
ctggcttctt | NM_008538 | myristoylated alanine rich protein kinase C substrate, Marcks | Ps | -4.76 |
Nil | NM_025958 | cullin-associated and neddylation-dissociated 2 (putative), Cand2 | Nil | -5.62 |
gctttgactgttctctt | NM_009870 | cyclin-dependent kinase 4, Cdk4 | -56.55 | -5.77 |
ctcagtaatg | AA122503 | EST from M2 cells of skin melanoma | -12.28 | -11.12 |
tggagcgttggctgtat | NM_009238 | SRY-box containing gene 4, Sox4 | -31.51 | -11.74 |
cacgacaccccccaccc | NM_007792 | cysteine and glycine-rich protein 2, Csrp2 | Ps | -26.10 |
tgtgtgaggtgttgtga | NM_009559 | zinc finger protein 57, Zfp57 | -16.33 | -32.74 |
gggacctcgtggaagcc | NM_010025 | doublecortin, Dcx | -24.56 | -82.11 |