Figure 2

Numerous changes in alternatively spliced mRNA isoforms induced by insulin and wingless. (a) Features of microarray design. The array contains 36-mer probes complementary to each exon and splice junction (sjnc) for all annotated Drosophila genes for which there is evidence of alternative splicing. The number of genes, mRNAs and probes present in the array are indicated. (b) Summary of regulated junctions and genes detected upon activation of insulin and wingless pathways. (c) Distribution of classes of alternative splicing events for all Drosophila genes (left) and for those regulated by insulin (middle) and wingless signaling (right). AFE, alternative first exon; ATE, alternative terminal exon; alt3(5)'ss, alternative 3(5)'splice site.