Figure 5

miR-17-5p targets MAPK9 translation. (a) Immunoblot analysis of miR-17-5p targets. Beta Actin (loading control), CCND1 and miR17-5p targets RBL2 and MAPK9 were assessed in untransfected, vector transfected and miR-17-5p transiently transfected lines. RBL2 and MAPK9 show lower protein levels while CCDN1 protein levels were dramatically increased in the miR17-5p expressing cell line. (b) Quantification of MAPK9 expression levels (assessed by immunoblot) in HEK293T-17-5p cell lines, and vector control cell lines grown under the same conditions. Mean and standard errors of independent experiments are shown (p = 0.02 in a Student's t-test).