Genomic analysis of the relationship between gene expression variation and DNA polymorphism in Drosophila simulans

Table 3 Gene feature length, polymorphism and divergence in females for genes with high, average and low levels of expression

	Low	Average	High	Tukey's HSD summary*	X ²	p-value^†
Number of genes	1,652	3,999	1,477
Length
EXON	1,877	1,825	1,319	L=A>H	198.71	***
5'UTR	287	241	213	L>A>H	15.53	0.0004
Intron	3,845	2,521	1,386	L>A>H	20.64	***
Number of introns	3.97	3.71	2.94	L=A>H	48.06	***
3'UTR	547	366	384	L>H=A	108.03	***
Polymorphism
CPR	0.0260	0.0304	0.0315	H=A>L	59.21	***
5'UTR	0.0110	0.0115	0.0094	A=L>H	28.06	***
Nonsynonymous	0.0028	0.0021	0.0013	L>A>H	341.18	***
Synonymous	0.0337	0.0325	0.0259	L=A>H	148.96	***
First intron	0.0283	0.0272	0.0240	L=A>H	19.94	***
All introns	0.0309	0.0298	0.0260	L=A>H	24.77	***
3'UTR	0.0136	0.0116	0.0089	L>A>H	106.22	***
Divergence^‡
CPR	0.0452	0.0550	0.0597	H>A>L	132.46	***
5'UTR	0.0218	0.0229	0.0209	A≥L≥H	6.70	0.0350
Nonsynonymous	0.0066	0.0048	0.0034	L>A>H	243.38	***
Synonymous	0.0513	0.0546	0.0476	A≥L≥H	79.80	***
First intron	0.0471	0.0459	0.0411	L=A>H	13.88	0.0010
All introns	0.0482	0.0489	0.0437	A=L>H	22.22	***
3'UTR	0.0241	0.0221	0.0168		74.98	***

*L, low expression; A, average expression; H, high expression (see Materials and methods). ^†X² and p-values derived from Kruskal Wallis; three asterisks denote p-value < 0.0001. ^‡Divergence refers to lineage specific divergence along the D. simulans branch.

ISSN: 1474-760X