Whole proteome identification of plant candidate G-protein coupled receptors in Arabidopsis, rice, and poplar: computational prediction and in-vivo protein coupling

Table 3 Results for the split-ubiquitin system protein-protein interaction assays between GPA1 Nub fusion proteins and the candidate GPCR-Cub-PLV fusion proteins

Results were recorded after 3-5 days of yeast growth on minimal media containing 1 mM methionine to identify proteins that interact specifically with GPA1 as indicated by the extent of yeast growth. (+) indicates moderate growth while (++) indicate heavy yeast growth. Empty vector control plates did not show growth (data not shown). X-Cub-PLV, candidate GPCR-Cub-PLV fusions. Nub_wt, wild type amino-terminal half of ubiquitin. NubG, reduced affinity mutant of Nub_wt.

ISSN: 1474-760X