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Table 3 Results for the split-ubiquitin system protein-protein interaction assays between GPA1 Nub fusion proteins and the candidate GPCR-Cub-PLV fusion proteins

From: Whole proteome identification of plant candidate G-protein coupled receptors in Arabidopsis, rice, and poplar: computational prediction and in-vivo protein coupling

   GPA1 Nub fusion orientations
Locus tested (X-Cub-PLV) Name Nubwt-GPA1 NubG-GPA1 GPA1-Nubwt GPA1-NubG
At1g57680.1 Cand1 ++ - ++ -
At3g05010.1 Cand2 ++ - + -
At3g59090.1 Cand3 ++ - ++ -
At3g59090.3 Cand5 ++ - ++ -
At5g18520.1 Cand7 ++ - ++ -
At5g27210.1 Cand8 ++ - - -
At4g21790.1 TOM1 ++ - ++ -
At4g30850.1 HHP2 ++ - + -
  1. Results were recorded after 3-5 days of yeast growth on minimal media containing 1 mM methionine to identify proteins that interact specifically with GPA1 as indicated by the extent of yeast growth. (+) indicates moderate growth while (++) indicate heavy yeast growth. Empty vector control plates did not show growth (data not shown). X-Cub-PLV, candidate GPCR-Cub-PLV fusions. Nubwt, wild type amino-terminal half of ubiquitin. NubG, reduced affinity mutant of Nubwt.