Whole proteome identification of plant candidate G-protein coupled receptors in Arabidopsis, rice, and poplar: computational prediction and in-vivo protein coupling

Table 2 Results for the split-ubiquitin system protein-protein interaction assays between candidate GPCR Nub fusion proteins and the GPA1-Cub-PLV fusion protein

		Candidate GPCR Nub fusion orientations
Locus tested	Name	Nub_wtX	Nub_GX	XNub_wt	XNub_G
At1g57680.1	Cand1	++	++	++	-
At3g05010.1	Cand2	++	++	++	-
At3g59090.1	Cand3	++	++	++	-
At3g59090.3	Cand5	++	+	++	-
At5g18520.1	Cand7	++	+	++	-
At5g27210.1	Cand8	++	++	+	-
At4g21790.1	TOM1	++	-	++	-
At4g30850.1	HHP2	++	+	-	-

All results were recorded after 3-5 of days of yeast growth on minimal media containing 1 mM methionine to identify proteins that interact specifically with GPA1 as indicated by the extent of yeast growth. (+) indicates moderate growth while (++) indicate heavy yeast growth. Empty vector control plates did not show growth (data not shown). Nub_wt, wild type amino-terminal half of ubiquitin. NubG, reduced affinity mutant of Nub_wt.

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