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Table 2 Results for the split-ubiquitin system protein-protein interaction assays between candidate GPCR Nub fusion proteins and the GPA1-Cub-PLV fusion protein

From: Whole proteome identification of plant candidate G-protein coupled receptors in Arabidopsis, rice, and poplar: computational prediction and in-vivo protein coupling

   Candidate GPCR Nub fusion orientations
Locus tested Name NubwtX NubGX XNubwt XNubG
At1g57680.1 Cand1 ++ ++ ++ -
At3g05010.1 Cand2 ++ ++ ++ -
At3g59090.1 Cand3 ++ ++ ++ -
At3g59090.3 Cand5 ++ + ++ -
At5g18520.1 Cand7 ++ + ++ -
At5g27210.1 Cand8 ++ ++ + -
At4g21790.1 TOM1 ++ - ++ -
At4g30850.1 HHP2 ++ + - -
  1. All results were recorded after 3-5 of days of yeast growth on minimal media containing 1 mM methionine to identify proteins that interact specifically with GPA1 as indicated by the extent of yeast growth. (+) indicates moderate growth while (++) indicate heavy yeast growth. Empty vector control plates did not show growth (data not shown). Nubwt, wild type amino-terminal half of ubiquitin. NubG, reduced affinity mutant of Nubwt.