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Table 1 Summary of pancreas SAGE libraries generated

From: Identification of transcripts with enriched expression in the developing and adult pancreas

Accession

Stage

Tissue subtype

Cell types represented

Library type

Tags sequenced*

Tag types

SM161/SM244

TS17

Pdx1 EGFP+†

All pancreas epithelial cells with the exception of rare Glucagon-positive cells

Long SAGElite

306,588

44,491

SM231

TS19

Pdx1 EGFP+

All pancreas epithelial cells with the exception of rare Glucagon-positive cells

Long SAGElite

317,716

49,572

SM162/SM245

TS20

Ngn3 EGFP-†

A mixture of pancreas cell types composed predominantly of mesenchymal cells and pancreas epithelial progenitors as well as those destined to become exocrine cell types

Long SAGElite

308,745

47,695

SM243/SM160

TS20

Ngn3 EGFP+

All endocrine progenitor cells as well as endocrine cells at various stages of maturation

Long SAGElite

320,473

51,847

SM225/SM249

TS21

Ngn3 EGFP+

All endocrine progenitor cells as well as endocrine cells at various stages of maturation

Long SAGElite

313,503

58,864

SM232

TS22

Ngn3 EGFP+

All endocrine progenitor cells as well as endocrine cells at various stages of maturation

Long SAGElite

301,222

37,726

SM223

TS22

Whole

A mixture of pancreas cell types composed predominantly of pancreas epithelial cells differentiating into exocrine cell types with some endocrine cells and mesenchymal cells

Long SAGE

98,189

13,676

SM016

TS26

Whole

A mixture of pancreas cell types composed predominantly of pancreas epithelial cells differentiating into exocrine cell types with some endocrine cells and mesenchymal cells

Long SAGE

81,130

17,963

SM102

DPN70

Isolated ducts

Hand picked adult ducts isolated by collagenase treatment and gradient centrifugation

Long SAGE

119,024

23,528

SM017

DPN70

Isolated islets

Hand picked adult islets isolated by collagenase treatment and gradient centrifugation composed of each of the major endocrine cell types

Long SAGE

99,968

16,039

  1. *After 95% quality cutoffs for all tags. †The Pdx1 EGFP and Ngn3 EGFP transgenic strains were obtained from Douglas Melton as described in Gu et al. [22]. DPN, days post natal.