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Table 1 Summary of genes implicated in gastric function in platypus

From: Loss of genes implicated in gastric function during platypus evolution

Protein Gene Status in platypus genome Confirmatory evidence
ATPase, H+/K+ exchanging, α polypeptide ATP4A Absent Southern blot
ATPase, H+/K+ exchanging, β polypeptide ATP4B Pseudogene PCR/direct sequencing
Cathepsin E CTSE Pseudogene PCR/direct sequencing
Gastrin GAST Absent Southern blot
Neurogenin 3 NGN3 Absent Southern blot
Pepsin A PGA Absent Southern blot/sequencing
Pepsin C PGC Absent Southern blot/sequencing
Gastric intrinsic factor GIF Present (expression pancreatic) RT-PCR
Chymosin CYMP Present (expression not detected) Sequencing/RT-PCR
  1. RT,-PCR, reverse transcription polymerase chain reaction.