The distribution of secondary structural content in observed and pseudo-clusters of FL8 and FL16. The statistical significance of the observed distribution can be estimated by comparing the respective plots for the pseudo-clusters. (a) helical; (b) β-strand; (c) turn; (d) irregular secondary structure. (e,f) Plots of normalized frequency of average percent of intra-hydrogen bonds (e), and percent relative side chain accessibility (f). The x- and y-axes of insets are the same as in the main figures, and depict information from the functionally important fragments with propensity ≥20 identified in this work.