Figure 8

Predicted structure and estimated spiked concentrations for the CD44 gene. (a) Structure of the CD44 gene spiked transcripts and probe positions represented by features. A gene feature is either an exon or a junction. Exon features are represented by F followed by the number of the corresponding exon and junction features are represented by the two exon features to which they belong joined by @ symbol. Each feature is made up of eight probes following a tiling strategy. As 23 features have been measured this makes a total of 184 probes. Probes corresponding to exon features F4, F5 and junction features F4@F5, F7@F10 do not match any of the spiked transcripts and therefore are not shown in (a). (b) Expected hybridization pattern of all probes for each of the two variants of CD44 gene. (c) Splicing structure prediction for CD44 gene applying the SPACE algorithm. (d) Estimated concentrations of the two variants of CD44 gene compared to spiked concentrations. The y-axis shows the predicted and actual concentration of each variant. The x-axis indicates the experiments and actual concentrations of each variant pair.