Figure 7
From: SPACE: an algorithm to predict and quantify alternatively spliced isoforms using microarrays
Experiment done with the CASP2 gene, transcripts Casp2L and Casp2S (synthetic data). Three arrays were performed with a concentration ratio between the two isoforms of CASP2 gene equal to 5:1 and another three with the opposite ratio 1:5. (a) Structure of the two transcripts of CASP2 gene and location of probes in the microarray. (b) Real structure of CASP2 gene indicated by probes. Probes that match perfectly are represented in white (100%), no hybridization in black (0%) and partial hybridization by different shades of gray (20%). (c) Predicted splicing structure for CASP2 gene with the alternating concentration ratio 5:1. If compared with the real structure of CASP2 transcripts (b), a strong similarity is noticed. (d) Real and estimated relative concentrations of the two isoforms of CASP2 gene in the experiment.