Figure 6

PCR-RFLP confirmation of the presence of serotype-specific chromosomes in three AD hybrid strains. Agarose gels are shown in which lane 1 for each contains size markers (1 kilobase [kb] ladder); lanes 2, 4, 6, 8, and 10 contain undigested PCR fragments; and the remaining lanes contain the same fragments after restriction enzyme digestion. (a) Digestion of PCR fragments (primers CNA01230 F/R) from chromosome (chr) 1 with AvaI (left panel) or StuI (right panel). (b) Digestion of PCR fragments (primers CNE04380 F/R) from chromosome 5 with TaqI (left panel) or HindIII (right panel). (c) Digestion of a PCR fragment (primers CNB01970 F/R) from chromosome 2 with NdeI (left panel) and a fragment (primers acidphos F/R) from chromosome 3 with TaqI (right panel).