Pathways affected in Daphnia magna after acute ibuprofen exposure. Presented is a simplified overview of pathways affected in D. magna following acute exposure to ibuprofen, showing a network of 21 interlinked genes based on Kyoto Encyclopedia of Genes and Genomes representing a stress-specific response. Expression of target genes is indicated by small arrows quantified by either microarrays after 24 hours of exposure (normal font) or quantitative PCR temporal expression based on 24 to 48 hours of exposure (bold font). GPX and CLECT are based on quantitative PCR validation data. Bold boxes and underlined genes represent the initiation point of the ibuprofen mode of action (see Figure 1). Abbreviations are as in Additional data file 1 and Figure 1 (see text for further details).