Proteomic analysis of the secretome of Leishmania donovani

Table 3 Highly enriched leishmania secreted proteins

GeneDB accession number	Protein identification^a	Mean Cm/CA ratio^b	Function^c	Predicted location^c
LmjF14.1360	Myo-inositol-1-phosphate synthase	3.93	Inositol biosynthesis [50]	Cytosol
LmjF23.0200	Endoribonuclease L-PSP (pb5), putative	3.91	Nuclease, mRNA cleavage	Cytosol
LmjF15.1203	60S acidic ribosomal protein P2	3.73	Translation [102]	Ribosome^d
LmjF35.2420	Phosphoinositide-binding protein, putative	3.51	Phosphoinositol binding, signal transduction	Cytosol^d
LmjF16.0140	Eukaryotic translation initiation factor 1A, putative	3.26	Translation	Cytosol, exosomes [24,38,39]
LmjF32.2180	Hypothetical protein, conserved	3.08	Translation initiation	Nucleus
LmjF11.0630	Aminopeptidase, putative, metallo-peptidase, Clan MF, Family M17	3.05	Proteolysis	Cytosol
LmjF35.3340	6-Phosphogluconate dehydrogenase, decarboxylating, putative	3.01	Glucose cataboloism	Cytosol
LmjF04.0310	Beta-fructofuranosidase, putative	2.93	Carbohydrate metabolism	Cytosol^d
LmjF36.3840	Glycyl tRNA synthetase, putative	2.87	Translation	Cytosol

^aThe Mascot algorithm {2008 609/id} was used to identify the protein names and the GeneDB accession numberes [29]. ^bMean of normalized, log normal (Ln) transformed conditioned medium (Cm)/cell associated (CA) peptide ratios for at least three of four experiments. ^cPutative functions and locations are derived from the GeneDB database {2007 238/id}unless otherwise noted. *Protein sequence contains an amino-terminal secretion signal peptide according to SignalP.

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