Figure 3
Ago proteins localize to cytoplasmic P-bodies. Human embryonic kindey cells (HEK 293T) were transfected with epitope-tagged Ago2 (FLAG/HA-Ago2). (a) Cells stained with DAPI to show the DNA. (b,c) The same cells stained with antibodies against (b) hemagglutinin (HA) and (c) Lsm4, a P-body marker involved in mRNA turnover. P-bodies are indicated by arrows. (d) Merged images from (b) and (c).