Skip to main content

Table 2 Protein data for the 54 protein isoforms identified in this study

From: Quantitative protein expression profiling reveals extensive post-transcriptional regulation and post-translational modifications in schizont-stage malaria parasites

Protein name PlasmoDB ID and NCBI GenBank accession number Calculated Spot number Mascot MS/MS ion search Average volume ratio Relative standard deviation Maximum fold change One-way ANOVA P value
   Mass (kDa) pI   Score Peptides matched Sequence coverage TP1 TP2 TP3 TP4 TP1 TP2 TP3 TP4 Median   
2-Cys peroxiredoxin [PDB:PF14_0368]
[Genbank:AAN36981]
22.0 6.7 - 559 4 48% 1.03 1.02 0.96 0.78 5.3% 15.4% 9.9% 6.4% 8.1% 1.33× 0.016
Actin-I [PDB:PFL2215w]
[Genbank:AAN36527]
42.1 5.2 1 533 9 40% 0.58 0.97 1.30 1.24 5.9% 5.9% 8.5% 3.2% 5.9% 2.24× <0.001
     2 609 8 37% 0.60 0.93 1.44 1.25 4.8% 5.6% 5.6% 3.4% 5.2% 2.39× <0.001
     3 391 8 36% 0.66 1.01 1.60 1.35 7.0% 4.4% 7.0% 2.2% 5.7% 2.43× <0.001
     4 415 5 21% 0.63 0.94 1.21 0.97 7.7% 3.4% 8.6% 6.8% 7.3% 1.91× <0.001
     5 192 6 29% 1.57 0.73 0.78 1.40 8.7% 7.0% 12.6% 11.3% 10.0% 2.15× <0.001
Adenosine deaminase [PDB:PF10_0289]
[Genbank:AAN35486]
42.9 5.4 - 484 7 23% 1.24 1.18 1.15 0.80 14.5% 5.0% 2.6% 6.6% 5.8% 1.55× <0.001
eIF4A/RNA helicase-1/helicase 45 [PDB:PF14_0655]
[Genbank:AAN37268]
45.3 5.5 1 318 8 24% 0.41 1.32 1.19 1.01 4.4% 3.7% 7.8% 5.8% 5.1% 3.26× <0.001
     2 159 2 10% 0.61 1.24 1.23 1.05 5.6% 5.0% 6.6% 2.7% 5.3% 2.03× <0.001
     3 143 6 22% 0.73 1.20 1.30 1.16 8.8% 6.1% 20.6% 9.5% 9.2% 1.79× <0.001
     4 133 4 19% 1.58 0.57 0.88 1.11 5.7% 5.2% 8.2% 3.6% 5.4% 2.78× <0.001
     5 242 4 13% 1.64 0.62 0.94 1.22 9.6% 6.5% 11.8% 10.4% 10.0% 2.65× <0.001
eIF4A-like helicase [PDB:PFB0445c]
[Genbank:AAC71878]
52.6 5.7 1 233 4 15% 0.99 1.05 1.24 1.22 7.9% 8.8% 15.7% 7.4% 8.3% 1.25× 0.024
     2 368 7 26% 1.02 1.06 1.18 1.13 7.8% 4.2% 7.6% 7.3% 7.4% 1.16× 0.045
eIF5A [PDB:PFL0210c]
[Genbank:AAN36131]
17.8 5.4 1 117 2 27% 0.12 0.32 1.26 1.79 18.2% 18.1% 10.9% 23.4% 18.2% 15.09× <0.001
     2 129 2 16% 1.05 0.98 0.79 0.55 4.9% 12.3% 30.1% 28.8% 20.5% 1.9× 0.005
     3 151 2 16% 1.27 1.44 1.09 0.65 19.4% 24.0% 16.9% 21.6% 20.5% 2.23× <0.001
Enolase [PDB:PF10_0155]
[Genbank:AAN35353]
48.6 6.2 1 344 10 30% 1.10 1.11 1.07 0.88 4.2% 4.4% 4.6% 7.2% 4.5% 1.26× <0.001
     2 513 10 33% 1.15 1.18 0.99 0.83 14.1% 9.5% 4.8% 10.0% 9.8% 1.42× <0.001
     3 627 10 33% 1.18 1.17 1.09 0.87 5.4% 5.0% 7.9% 8.5% 6.7% 1.35× <0.001
     4 897 10 39% 1.13 1.06 1.11 0.92 11.7% 2.2% 8.3% 5.2% 6.7% 1.22× 0.016
     5 138 4 15% 1.03 0.91 1.14 0.93 19.9% 7.8% 9.9% 11.9% 10.9% 1.25× 0.125
     3a 213 5 14% 1.83 1.09 0.68 0.52 12.7% 12.9% 24.3% 3.3% 12.8% 3.52× <0.001
     4a 122 3 11% 1.43 1.06 0.85 0.73 0.9% 3.1% 16.0% 4.8% 4.0% 1.94× <0.001
EXP-2 [PDB:PF14_0678]
[Genbank:AAN37291]
33.6 5.1 - 133 3 12% 0.27 0.70 0.88 0.97 8.1% 4.3% 10.4% 9.3% 8.7% 3.62× <0.001
HSP40 [PDB:PFE0055c]
[PDB:MAL5P1.12]
[Genbank:CAD51377]
47.8 8.1 - 118 4 19% 0.70 0.85 0.94 2.06 13.2% 37.4% 14.2% 18.9% 16.5% 2.94× <0.001
HSP60 [PDB:PF10_0153]
[Genbank:AAN35351]
62.9 6.7 1 171 5 11% 1.29 0.96 1.10 1.37 8.6% 4.1% 7.0% 3.8% 5.5% 1.43× <0.001
     2 235 5 11% 1.31 0.89 1.08 1.35 5.9% 3.8% 5.5% 6.5% 5.7% 1.51× <0.001
HSP70-1a [PDB:PF08_0054]
[Genbank:CAD51185]
74.4 5.5 1 607 10 20% 1.31 1.13 1.01 0.93 14.7% 10.1% 12.9% 7.9% 11.5% 1.41× 0.008
     2 644 10 22% 1.46 1.23 1.06 0.96 5.7% 4.5% 10.8% 7.1% 6.4% 1.52× <0.001
HSP70-2a [PDB:PFI0875w]
[Genbank:CAD51861]
72.5 5.2 1 486 8 17% 0.95 1.15 0.98 1.08 8.7% 5.0% 7.8% 8.1% 8.0% 1.2× 0.014
     2 724 10 23% 1.06 1.07 1.02 1.11 8.6% 4.8% 7.2% 4.4% 6.0% 1.08× 0.369
     3 694 10 21% 1.26 0.87 1.03 1.09 4.9% 2.5% 6.1% 4.1% 4.5% 1.46× <0.001
HSP70-3a [PDB:PF11_0351]
[Genbank:AAN35935]
73.7 6.5 1 193 5 7% 1.08 0.97 0.97 1.06 7.2% 7.8% 16.2% 4.9% 7.5% 1.11× 0.299
     2 488 8 12% 1.01 0.98 1.16 1.11 8.0% 2.2% 11.3% 6.2% 7.1% 1.19× 0.028
CA1 - [Genbank:NP_001729] 28.9 6.6 1 264 5 32% 1.48 0.98 0.60 0.59 6.2% 4.3% 13.7% 3.8% 5.2% 2.5× <0.001
     2 412 7 40% 1.16 0.94 0.72 0.77 6.0% 7.1% 17.4% 6.0% 6.6% 1.61× <0.001
Human SOD1 - [Genbank:NP_000445] 16.2 5.7 - 247 5 57% 1.04 1.18 1.13 0.74 9.2% 12.5% 7.3% 16.2% 10.8% 1.6× 0.001
HETK [PDB:PFF1335c]
[PDB:MAL6P1.153]
[Genbank:CAG25088]
20.6 7.0 - 575 8 56% 1.02 1.13 0.96 0.74 1.9% 9.0% 5.9% 7.5% 6.7% 1.53× <0.001
Hypothetical protein PF10_0325 [PDB:PF10_0325]
[Genbank:AAN35522]
33.2 5.6 1 380 7 33% 1.08 1.11 1.04 0.93 1.5% 7.0% 11.7% 14.8% 9.4% 1.2× 0.107
     2 497 8 33% 1.13 1.09 0.99 0.88 5.8% 4.9% 18.7% 17.9% 11.9% 1.28× 0.097
Hypothetical protein PFF1295w [PDB:PFF1295w]
[Genbank:CAG25080]
43.9 7.2 - 245 5 20% 1.06 1.04 0.95 0.91 2.2% 7.1% 13.7% 5.0% 6.0% 1.16× 0.072
Hypothetical protein PFI1270w [PDB:PFI1270w]
[Genbank:CAD51940]
24.9 5.5 1 332 6 21% 1.31 0.98 1.01 1.00 11.6% 8.7% 10.1% 14.3% 10.8% 1.34× 0.012
     2 74 3 17% 0.93 0.97 1.36 1.65 8.6% 16.8% 11.6% 9.8% 10.7% 1.77× <0.001
M1AP [PDB:MAL13P1.56]
[Genbank:CAD52253]
126.6 7.3 - 171 7 10% 1.47 0.91 0.87 0.89 12.2% 8.7% 13.4% 10.9% 11.5% 1.69× <0.001
OAT [PDB:PFF0435w]
[PDB:MAL6P1.91]
46.0 6.5 - 589 9 29% 1.20 1.05 1.06 1.06 0.9% 5.8% 12.0% 5.4% 5.6% 1.14× 0.116
PEMT [PDB:MAL13P1.214]
[Genbank:CAD52560]
31.3 5.4 1 790 9 46% 1.07 1.12 1.09 0.89 7.2% 10.3% 9.6% 16.7% 9.9% 1.26× 0.049
     2 182 3 14% 0.76 0.97 1.13 1.05 6.2% 5.8% 14.0% 16.3% 10.1% 1.49× 0.003
Proteasome component C8 [PDB:PFC0745c]
[Genbank:CAB11152]
29.7 6.4 1 67 1 7% 1.17 1.20 1.12 0.83 12.9% 3.1% 5.1% 7.6% 6.4% 1.44× <0.001
     2 275 4 28% 0.98 0.96 0.96 0.82 7.4% 9.2% 10.8% 11.2% 10.0% 1.2× 0.072
TCP1a [PDB:PF11_0331]
[Genbank:AAN35915]
60.6 6.7 - 110 5 11% 1.05 0.97 1.04 1.04 3.0% 4.1% 6.4% 6.2% 5.1% 1.08× 0.190
TPI [PDB:PF14_0378]
[Genbank:AAN36991]
28.1 6.0 1 579 8 43% 1.18 1.13 1.04 0.86 6.1% 4.5% 11.8% 9.4% 7.7% 1.37× <0.001
     2 578 8 45% 1.20 1.11 1.07 0.88 7.0% 5.1% 10.0% 9.6% 8.3% 1.37× <0.001
Uridine phosphorylase [PDB:PFE0660c]
[Genbank:CAD51497]
27.5 6.1 - 430 7 35% 1.23 1.16 1.09 0.73 5.2% 3.6% 7.0% 6.5% 5.8% 1.68× <0.001
  1. The average volume ratios represent log2-transformed values and have not been mean-centered around zero. aNomenclature in agreement with Shonhai and coworkers [35]. CA1, human carbonic anhydrase 1; eIF, eukaryotic initiation factor; EXP, exported protein; HETK, hydroxyethylthiazol kinase; HSP, heat shock protein; M1AP, M1-family aminopeptidase; OAT, ornithine aminotransferase; PEMT, phosphoethanolamine N-methyltransferase; SOD, superoxide dismutase; TCP1a, T-complex protein 1, α subunit; TPI, triose phosphate isomerase.