Figure 5

Role of Egr1 in apoptosis. (a) Growth curve of M12 cells over a period of 72 h, with or without UV-C irradiation. The experiments were performed in triplicates and the error bars represent the data from the triplicate experiments (b) Western blot analysis of M12 cells treated with UV-C, and collected at varying time points after UV irradiation. Anti-PARP (to demonstrate apoptosis) was used to identify PARP cleavage product (this antibody only detects the cleaved product and not the native protein). (c) M12 cells were treated with SiGenome control and SiEgr1 and followed by UV-C stimulation. Cells were collected after 24 h of UV treatment, and western blot analysis, using anti-PARP, showed that Egr1 is involved in apoptosis of M12 cells.