Figure 3

Comparative analysis of Yap1-mediated transcriptional modules. (a) Genes up-regulated during the time course of benomyl treatment were assigned to two groups as a function of their regulation by the transcription factors ScYap1p in S. cerevisiae (ScYap1p-dependent genes) and Cgap1p in C. glabrata (Cgap1p-dependent genes). In S. cerevisiae, the ScYap1p transcription factor accounts for 41% of the genes induced during benomyl stress, whereas, in C. glabrata, the transcription factor Cgap1p accounts for 24% of the genes induced during the benomyl stress response. (b) Eight versions of the YRE have been described in previous studies (TGACTCA [39], TGACTAA[38], TTACTAA[38], TTAGTAA [37], TTAGTCA[38], TGACAAA[40], TGAGTAA [40]and TTACAAA [40]). We looked for these motifs in the upstream regions (from nucleotides -600 to -1, direct strand) of up-regulated genes during the benomyl stress response. The percentages of genes with a YRE in their promoter are shown here. In S. cerevisiae, the motifs TTACTAA and TTAGTAA appeared to be the more frequent in the promoters of genes regulated by ScYap1p, whereas in C. glabrata, the motifs TTAGTAA and TTACAAA appeared to be the more frequent in Cgap1p-dependant genes.