Table 2 Bacteria
E. coli strains | Genotype | Source and/or reference |
|---|---|---|
ER1562 | F- λ-endA1 thi-1 supE44 hsdR2 mcrB1 mcrA1272::Tn10 | New England Biolabs [89] |
ER1563 | F- λ-endA1 thi-1 supE44 hsdR2 mcrA1272::Tn10 | New England Biolabs [89] |
BIK18046 | ER1562 but Tcs | Tcs with fusaric acid |
BIK18051 | ER1563 but Tcs | Tcs with fusaric acid |
BIK18116 | ER1562 Δ(recB-recC)::kan | KmR with pKD46-mediated transformation with PCR product from deletion allele primers and pKD13 template |
BIK18118 | ER1563 Δ(recB-recC)::kan | KmR with pKD46-mediated transformation with PCR product from deletion allele primers and pKD13 template |
BIK18120 | ER1562 ΔrecA::kan | KmR with pKD46-mediated transformation with PCR product from deletion allele primers and pKD13 template |
BIK18125 | ER1563 ΔrecA::kan | KmR with pKD46-mediated transformation with PCR product from deletion allele primers and pKD13 template |
BIK18142 | ER1562 ΔaraBAD::kan | KmR with pKD46-mediated transformation with PCR product from deletion allele primers and pKD13 template |
BW27269 | lacIqrrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 | E. coli Genetic Stock Center [52] |
|  | Δ(rhaBAD)568Δ(araFGH)::kan903 |  |
BW27535 | lacI q rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 | E. coli Genetic Stock Center [52] |
|  | Δ(rhaBAD) 568 g(ΔaraEp kan Pcp13-araE) |  |
BIK18244 | BIK18046 ΔaraBAD::kan | P1 from BIK18116 to ER1562 |
BIK18246 | BIK18051 ΔaraBAD::kan | P1 from BIK18116 to ER1563 |
BIK18248 | BIK18046 ΔaraBAD | BIK18244 Kms with pCP20 |
BIK18249 | BIK18051 ΔaraBAD | BIK18246 Kms with pCP20 |
BIK18250 | BIK18046 ΔaraBAD ϕ(ΔaraEp kan Pcp13-araE) | P1 from BW27535 to BIK18248 |
BIK18252 | BIK18051 ΔaraBAD ϕ(ΔaraEp kan Pcp13-araE) | P1 from BW27535 to BIK18249 |
BIK18254 | BIK18046 ΔaraBAD ϕ(ΔaraEp Pcp13-araE) | BIK18250 Kms with pCP20 |
BIK18255 | BIK18051 ΔaraBAD ϕ(ΔaraEp Pcp13-araE) | BIK18252 Kms with pCP20 |
BIK18256 | BIK18046 ΔaraBAD ϕ(ΔaraEp Pcp13-araE) Δ(araFGH)::kan903 | P1 from BW27269 to BIK18254 |
BIK18258 | BIK18051 ΔaraBAD ϕ(ΔaraEp Pcp13-araE) Δ(araFGH)::kan903 | P1 from BW27269 to BIK18255 |
BIK18260 | BIK18046 ΔaraBAD ϕ(ΔaraEp Pcp13-araE) Δ(araFGH) | BIK18256 Kms with pCP20 |
BIK18261 | BIK18051 ΔaraBAD ϕ(ΔaraEp Pcp13-araE) Δ(araFGH) | BIK18258 Kms with pCP20 |
BIK18282 | BIK18260 ΔrecA::kan | P1 from BIK18120 to BIK18260 |
BIK18284 | BIK18261 ΔrecA::kan | P1 from BIK18120 to BIK18261 |
BIK18286 | BIK18260 Δ(recB-recC)::kan | P1 from BIK18116 to BIK18260 |
BIK18288 | BIK18261 Δ(recB-recC)::kan | P1 from BIK18116 to BIK18260 |
BIK18290 | BIK18260 ΔrecA | BIK18282 Kms with pCP20 |
BIK18291 | BIK18261 ΔrecA | BIK18284 Kms with pCP20 |
BIK18292 | BIK18260 Δ(recB-recC) | BIK18286 Kms with pCP20 |
BIK18293 | BIK18261 Δ(recB-recC) | BIK18288 Kms with pCP20 |
DH5α | F- λ- ϕ 80 dlacZ ΔM15Δ(lacZYA-argF)U169 deoR | Laboratory collection [91] |
| Â | recA1 endA1 hsdR17 phoA supE44 thi-1 gyrA96 relA1 | Â |
DH5α MCR | DH5α Δo(mrr-hsdRMS-mcrBC) | S Ohta [92] |
DH10B | F- araDJ39 Δ(ara, leu)7697 ΔlacX74 galU galK rpsL | Laboratory collection [92] |
|  | deoR ϕ 80 dlacZΔM15 endA1 nupG recAl mcrA |  |
|  | Δo(mrr-hsdRMS-mcrBC) |  |
JWK1944_2 | lacIqrrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 Δdcm::kan | National Institute of Genetics [116] |
BIK18308 | DH10B Δdcm::kan | P1 from JW1944-2 to DH10B |
BMH71-18 mutS | Δ(lac-proAB) supE thi-1 mutS215::Tn10/F' [traD36 proAB+ lacIq lacZΔM15] | TaKaRa Bio |
JC8679 | F- λ- supE44 thr-1 ara-14 leuB6 Δ(gpt-proA)62 lacY1 | AJ Clark [117] |
| Â | tsx-33 galK2 hisG4 rfbD1 mgl-51 rpsL31 kdgK51 xyl-5 | Â |
| Â | mtl-1 argE3 thi-1 recB21 recC22 sbcA23 | Â |
BIK1421 | JC8679 mutS215::Tn10 | P1 from BMH71-18 mutS to JC8679 |
GW2730 | thr-1 leu-6 his-4 argE-3 galK2 strA31 ilvts tif-1 sfiA11 | GC Walker [118] |
|  | ΔlacU169 lexA71::Tn5 |  |
BIK1016 | MC1060 (pCHR38) | C Sasakawa [119] |
BIK1185 | GW2730 but lexA71::Tn5-Gm | Central part of Tn5 in GW2730 was replaced by Gm |
|  |  | BIK1016 × GW2730 |
GC2597 | sfiA::Tn5 pyrD thr leu his lac gal malB srl::Tn10 | National Institute of Genetics [120] |
| Â | sfiC str | Â |
BIK1218 | JC8679 lexA3(Ind-) malF::Tn10 | N Takahashi [121] |
BIK18262 | BIK18260 mutS215::Tn10 | P1 from BIK1421 to BIK18260 |
BIK18264 | BIK18261 mutS215::Tn10 | P1 from BIK1421 to BIK18261 |
BIK18270 | BIK18260 malF::Tn10 | P1 fromBIK1218 to BIK18260 |
BIK18271 | BIK18260 lexA3(Ind-) malF::Tn10 | P1 fromBIK1218 to BIK18260 |
BIK18275 | BIK18261 malF:: Tn10 | P1 fromBIK1218 to BIK18261 |
BIK18276 | BIK18261 lexA3(Ind-) malF::Tn10 | P1 fromBIK1218 to BIK18261 |
BIK18266 | BIK18260 sulA::Tn5 | P1 from GC2597 to BIK18260 |
BIK18268 | BIK18261 sulA::Tn5 | P1 from GC2597 to BIK18261 |
BIK18278 | BIK18260 sulA::Tn5 lexA71::Tn5-Gm | P1 fromBIK1185 to BIK18266 |
BIK18280 | BIK18261 sulA::Tn5 lexA71::Tn5-Gm | P1 fromBIK1185 to BIK18268 |