Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations

Table 1 cDNA sequence evidence used in the present study

ESTs (conventional cDNA clones)*	1,179,850
5' EST	589,329
3' EST	590,521
5'-full-length ESTs	202,535
Oligo-capping cDNA library-derived ESTs^†	2,079
Spliced-leader mRNA derived ESTs^‡	199,947
5'-RACEs from oligo-capping cDNA pool^§	509
Full insert cDNA sequences^¶	8,877

*There were 672,390 ESTs published before [1, 12]. The rest of the ESTs were produced recently and high quality reads among them were deposited in the GeneBank database ([GenBank: FF685517-FF836289] and [GenBank:FF848360-FG007279]). ^†Described in [2]. ^‡Pooled data from two sets of SL-based reverse-transcription PCR analyses. One dataset consisted of 19,571 sequences derived from oligo(dT)-primed cDNA of mRNA from pooled embryonic/adult stages and several adult tissues (Y Satou et al., unpublished data). The other consisted of 180,376 SL-containing sequences >30 nucleotides derived from random-hexamer-primed cDNA of mRNA from tailbud embryos (J Matsumoto et al, manuscript in preparation). ^§From a study by oligo-capping 5'-RACE for determining 5'-ends of mRNAs encoding transcription factors (Y Satou et al., unpublished data). ^¶Sequences of full-inserts of cDNA clones downloaded from the public database.

ISSN: 1474-760X