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Table 1 Phosphopeptides identified from in vitro phosphorylated GST-Rb

From: Identification of CDK2 substrates in human cell lysates

Peptide sequence

SP/TP sites

R.GSTRPPTLS*PIPHIPR.S†

S780

R.GSTRPPTLSPIPHIPRS*PYK.F†

S788

R.SPYKFPSS*PLR.I

S795

R.IPGGNIYIS*PLKSPYK.I

S807

R.IPGGNIYISPLKS*PYK.I

S811

R.IPGGNIYIS*PLKS*PYK.I

S807, S811

K.ISEGLPT*PTK.M

T821

K.ISEGLPT*PTKMTPR.S

T821

K.ISEGLPTPTKMT*PR.S

T826

K.ISEGLPT*PTKMT*PR.S

T821, T826

  1. Phosphorylation sites identified with highest probability are marked by boldfaced letters and asterisks to their right. The other boldfaced serine(s) and threonine(s) within the same peptide are potential alternative phosphorylation sites for the ones marked with the asterisk due to the ambiguity in assigning the exact site(s) of phosphorylation. †These are unnatural peptides containing partial sequences of Rb protein and GST fusion.
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Genome Biology

ISSN: 1474-760X

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