Figure 5

Sensitivity trends of SeqFEATURE, Gene3D, Pfam, and HMMPanther at low sequence identities. We compared the sensitivity of SeqFEATURE at three specificity cutoffs against the sensitivity of Gene3D, Pfam, and HMMPanther on test sets filtered for low sequence identity. We evaluated each method on the subset of the original test set that had less than the specified sequence identity to the training sets. As sequence identity decreases, the sequence-based methods show a clear trend towards lower sensitivity. In contrast, SeqFEATURE at all three cutoffs shows no such downward trend, indicating robust detection of function even when sequence identity is very low.