Structural profile plot for expression level for six control genes, Calb1, Cst3, Dkk3, Gad1, Nov, and Pvalb. (a) Expression levels with error bars for 4 days × 4 probe measurements after three-dimensional anatomic mapping. (b) Original ISH images at mid-sagittal plane. (c) Segmentation expression mask of corresponding ISH sections (b) used as the basis for quantification of expression level and density. CB, cerebellum; CTX, cortex; HIP, hippocampus; HY, hypothalamus; MB, midbrain; MD, medulla; OLF, olfactory bulb; P, pons; PAL, pallidum; STR, striatum; TH, thalamus; RHP, retrohippocampal region.