Figure 2

Maximum likelihood trees for ACE1 cluster genes and their homologs. (a) ACE1 and SYN2; (b) RAP1 and RAP2; (c) CYP1 and CYP4; (d) CYP2 and CYP3; (e) ORF3; (f) ORFZ. In each tree, genes that appear in Figure 1 are named in color or bold black. Yellow highlighting shows the five genes in the A. clavatus ACE1 cluster whose closest relatives are genes from part B of the M. grisea cluster. Bootstrap percentages are shown for all nodes. Trees were constructed from amino acid sequences as described in Methods using PHYML after alignment with ClustalW and Gblocks filtering. Trees for the other five genes in the ACE1 cluster are shown in Additional data file 1. The values of the shape parameter (α) for the gamma distribution were estimated from the data as 1.329, 1.441, 2.476, 2.615, 2.536 and 0.961 for panels a-f, respectively. The proportions of invariant sites are 0.028, 0.035, 0.030, 0.068, 0.000 and 0.000, respectively. The M. grisea SYN2 gene corresponds to parts of the automatically-annotated gene models MGG_12452.5 and MGG_12451.5.