Figure 8

Analysis of adipocyte and macrophage associated markers in BMP2⁺ cells. (a) RT-PCR analysis of adipocyte specific markers. (b) Relative expression levels of the genes presented in (a) as obtained by Affymetrix analysis. The expression levels of each gene were normalized with its maximum level set as 100%. Each result is an average of three independent experiments (Additional data file 13). (c) Sudan red staining on 11-day-old puromycin untreated culture after plating the BMP2⁺ cells (7 + 11, 18 days in total). (d) F4/80 immunuostaining on 18-day-old puromycin untreated culture after plating BMP2⁺ cells (7 + 18, 25 days in total).