Figure 4

Detection of smooth muscle cells after differentiation of the BMP2⁺ cells. (a) Expression of SMA in the BMP2⁺ cells detected by qRT-PCR. (b) Microarray relative expression levels of various smooth muscle specific genes in BMP2⁺ cells. The expression levels of each gene were normalized with its maximum level set as 100%. Each result is an average of three independent experiments (Additional data file 13). (c) Detection of smooth muscle cells 1 day after plating the BMP2⁺ cells (7 + 1 days in total; top left), 8 days after plating with puromycin (7 + 8 days in total; top right), 18 days after plating with puromycin (7 + 18 days in total; bottom left) and 18 days after plating without puromycin (7 + 18 days in total; bottom right).