Figure 3

Analysis of neural crest stem cell associated transcripts in BMP2⁺ cells. (a) RT-PCR analysis for the representative genes associated with NCSCs. (b) Relative expression level of the genes presented in (a) as obtained by Affymetrix analysis. The expression levels of each gene were normalized with its maximum level set as 100%. Each result is an average of three independent experiments (Additional data file 13). (c) Detection of p75 and GFAP in BMP2⁺ cells labelled by immunohistochemistry. Immunuostainings with anti-p75 (left panel) and anti-GFAP (right panel) to show the presence of NCSCs and the astrocytes, respectively, in BMP2⁺ cells one day after plating (7 + 1 days in total).