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Table 2 Relative transcript levels* of repeats in ES cell lines

From: The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells

  Minor satellite Major satellite X141
WT (OS25) 0.4 ± 0.1 1.8 ± 0.5 1.8 ± 0.3
Eed KO 7.2 ± 6.5 18.5 ± 17.0 1.8 ± 0.2
Dnmt1 KO 0.3 ± 0.1 2.1 ± 0.0 2.0 ± 1.3
Dnmt3a/3b DKO 0.1 ± 0.0 5.7 ± 2.4 1.5 ± 0.9
G9a WT 0.1 ± 0.0 0.7 ± 0.1 0.5 ± 0.7
G9a KO 0.4 ± 0.4 1.0 ± 0.2 ND
Suv39 h1/h2 WT 4.9 ± 0.8 2.5 ± 1. 6 1.1 ± 0.3
Suv39 h1/h2 DKO 4.0 ± 1.3 2.2 ± 0.7 2.1 ± 0.5
Dicer WT 0.2 ± 0.0 4.1 ± 0.0 2.7 ± 0.0
Dicer KO 0.9 ± 0.6 17.4 ± 5.5 4.1 ± 1.2
C2C12 dif 100 100 100
  1. *Levels of repeat sequence RNA were normalized to a house keeping gene (Ube) and expressed as a percentage of the levels detected in differentiated mouse myocytes derived from C2C12 (C2C12 dif), samples in which high levels of major and minor satellite transcripts have previously been detected [56]. Controls without reverse transcriptase were analyzed in parallel to dismiss contamination with genomic DNA.
  2. Four independent samples showed variable but increased transcript levels: major satellite, 8.2%, 8.7%, 13.4%, and 43.8% of C2C12; minor satellite, 2.6%, 7.1%, 11.8%, and 13.8% of C2C12. ND, none detected.