Figure 6

GFP reporters validate neuronal microarray datasets. Transgenic animals expressing GFP reporters for representative genes detected in neuron-enriched microarray datasets. Anterior to left, ventral down. GFP images are combined with matching DIC micrographs for panels (b-g). (a,e) mec-12::GFP is expressed in touch neurons (arrow) and in specific ventral cord motor neurons (e) at the L2 stage. (b,c) tsp-7::GFP and C04E12.7::GFP are widely expressed in the nervous system with bright GFP in head and tail ganglia and in motor neurons of the ventral nerve cord (arrow heads). (d,f,g,h) Note expression of GFP reporters for sto-4, nca-1, and syg-1 in A-class (DA, VA) and in other ventral cord motor neurons (for example, DB, VB).