Figure 2

Schematic of the microarray experiment to determine half-lives through the life cycle. Four separate time course experiments were conducted at 12-hour intervals using a single source culture of synchronized parasites. Numbers in red represent the hour after invasion when actD was added in relationship to the previously published transcriptome experiment. Total RNA was subsequently harvested at the indicated time points. These samples were reverse transcribed into cDNA and hybridized to DNA microarrays. Specific spiked controls were included to determine correct normalization during microarray scanning.