Figure 2

Line means for two typical transcripts across the NC sample. Each plot shows the mean relative fluorescence intensity on a log base-2 scale for the four samples (two control and two nicotine-treated) of each line in random order (± 1 standard deviation unit). (a) CG7843 (unknown gene that is predicted to be involved in defense/toxin response) is an example of a gene with bimodal abundance, with the minor transcript abundance class centered approximately fourfold more abundant than the average transcript on the array (relative fluorescence intensity = +2), and the major transcript abundance class (TAC) twofold less abundant than the average (relative fluorescence intensity = -1). (b) CG12141 (encoding Lysyl tRNA synthetase) is a gene with a single mode of transcript abundance, given the variance among and within lines.