Figure 5

Analysis of new human lipase genes. (a) Schematic representation of the lipase gene cluster on chromosome 10q23.31. Six lipase genes, including the newly described LIPL1 (LIPJ), LIPL2 (LIPK), LIPL3 (LIPM) and LIPL4 (LIPN), form a cluster also containing four unrelated genes. (b) Alignment of the protein sequences of the six human lipases from the chromosome 10q23.31 cluster. The amino acids of the catalytic triad are boxed. The alignment was generated with Multalin software [71]. (c) Hierarchical clustering of human and mouse abhydro-lipase gene family members. The human LIPA, LIPF, LIPK, LIPM and LIPN, but not LIPJ, proteins have clear mouse orthologues (lower-case gene names). The six hypothetical mouse genes found in place of the LIPJ gene (Lipdc1-5) form a separate branch of the phylogenetic tree. This tree was generated with the Tree Top software [72]. Bootstrap values are indicated in red.