Table 2 Sequences used to replace wild-type sequence in shha and shhb ar-Cs to generate the specified reporter constructs
Construct name | Wild-type sequence | Mutated/introduced sequence |
|---|---|---|
0.8shha:gfp:z-shha-arCΔC1 | TGCACCTGAGCAAATA | GTACAAGTCTACCCGT |
0.8shha:gfp:z-shha-arCΔC2 | GAAGTGTCCTTTTCCAAGAGT | TCCTGTAAGCCCAAGCTCTAC |
0.8shha:gfp:z-shha-arCΔC3 | AATGACAATGTCC | CCGTCACCGTGAA |
0.8shha:gfp:z-shha-arCΔC4 | CTTTATTGGTTTTTAATTAGA | AGGGCGGTTGGGGGCAGGCGG |
0.8shha:gfp:z-shha-arC+C4m | CTTTATTGGTTTTTAATTAGA | CTTTATTGAGTTTTTTTAAATTAAGG |
0.8shha:gfp:z-shhb-arCΔC1 | TGCACCTGTGTAAACA | GTACAAGTCTACCCGT |
0.8shha:gfp:z-shhb-arCΔC3 | TTTAAATGACAATGTCT | GGCTCCGTCACCGTGAA |
0.8shha:gfp:z-shhb-arC+C2 | CAGGGAAAAGCACAGTCTGT | GAAGTGTCCTTTTCCAAGAGT |
0.8shha:gfp:z-shhb-arC+C4 | GACTTTGTGTAAATTCAGCAG | CTTTATTGGTTTTTAATTAGA |
0.8shha:gfp:z-shhb arC+C2rnd | CAGGGAAAAGCACAGTCTGT | TCTCCAGGCTCAACCATGAGC |
0.8shha:gfp:z-shhb-arC+C4rnd | GACTTTGTGTAAATTCAGCAG | AGAAAGCTCGCGCGACCATGA |