RAD marker microarrays enable rapid mapping of zebrafish mutations

Table 1 Zebrafish RAD marker microarray characterization

	Number of array elements	AB-specific elements	WIK-specific elements	Total polymorphic elements
AB Library	6,144	1,373	14	1,387
WIK Library	7,680	32	1,709	1,741
Both Libraries	13,824	1,405	1,723	3,128 (22.6%)

Restriction site associated DNA (RAD) tags from AB and WIK were fluorescently labeled and competitively hybridized directly against each other to the RAD marker microarray. Elements with a differential hybridization greater than twofold were counted as polymorphic.

ISSN: 1474-760X